General Cell Based Assays
Eurofins Discoverx PathHunter Pertuzumab Bioassay Kit, 2-Plate. (93-1042Y3-00095)
The PathHunter Pertuzumab Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses heregulin-β2-induced dimerization of ErbB2 and EGFR receptors. This product uses EFC technology. The cell line included overexpresses ErbB2 target protein fused to PK alongside ErbB3 target protein fused to EA. Upon ligand-induced dimerization of ErbB2 and ErbB3, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress MC3R Total Internalization Assay, 2pl Kit. (93-0837E3CP0M)
The PathHunter MC3R Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of MC3R via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged MC3R and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the MC3R receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HTR1F U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0699E3CP0L)
The PathHunter HTR1F eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR1F GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR1F and EA-tagged β-Arrestin-2. Activation of HTR1F stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress VIPR2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0317E2CP0M)
The PathHunter VIPR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. VIP) based activation of VIPR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged VIPR2 and EA-tagged β-Arrestin-2. Activation of VIPR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CHRM1 U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0859E3CP0L)
The PathHunter CHRM1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. acetylcholine) based activation of CHRM1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CHRM1 and EA-tagged β-Arrestin-2. Activation of CHRM1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery MAP Kinase WCL20ate
The MAP Kinase Whole Cell Lysate Kit from Meso Scale Discovery provides reagents to detect total and phosphorylated ERK1/2 JNK1/2 and p38 MAP kinases in cell lysates The kit contains a 96-well plate coated with capture antibodies detection antibodies and buffers needed to perform the assay The electrochemiluminescent detection allows for high sensitivity and a wide dynamic range This kit allows for the quantitative analysis of MAP kinase activation in cells With a single 96-well plate up to 40 samples can be analyzed in triplicate
Eurofins Discoverx PathHunter eXpress mGHSR1a CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0605E2MCP2M)
The PathHunter mGHSR1a eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. grehlin) based activation of mGHSR1a GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mGHSR1a and EA-tagged β-Arrestin-2. Activation of mGHSR1a stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human S100A8/MRP8 ASY
The R-PLEX Human S100A8 (MRP8) Assay Kit from Meso Scale Diagnostics is a multiplex immunoassay that measures S100A8 protein levels in human serum plasma and cell culture supernatants The assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and requires low sample volume The kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer sample diluent and calibrators and controls The assay has a typical sensitivity of 0 3 pg/mL and an upper limit of quantification of 10 000 pg/mL It is for research use only
American Research Products Inc PrEST Antigen MPO
PrEST Antigen MPO antigen APrEST87920 for myeloperoxidase Recombinant protein fragment of Human MPO
Cellular Dynamics International iCell DopaNeurons, Cryopreserved, 3 vials, > 1 x 10^6 cells/vial, Fully differentiated, Human, Midbrain floorplate, >80% TH+, ≥90% MAP2+, ≥50% FoxA2+, ≥50% TH+, Branched networks ≤ 3 days
iCell DopaNeurons, Cryopreserved, 3 vials, > 1 x 10^6 cells/vial, Fully differentiated, Human, Midbrain floorplate, >80% TH+, ≥90% MAP2+, ≥50% FoxA2+, ≥50% TH+, Branched networks ≤ 3 days
American Research Products Inc PrEST Antigen VIPR2
PrEST Antigen VIPR2 antigen APrEST86375 for vasoactive intestinal peptide receptor 2 Recombinant protein fragment of Human VIPR2
Eurofins Discoverx PathHunter eXpress CALCRL-RAMP1 (CGRP1) CHO-K1 β-Arrestin GPCR Assay, 2pl Kit. (93-0269E2CP0M)
The PathHunter CALCRL-RAMP1 (CGRP1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. β-CGRP) based activation of CALCRL GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CALCRL and EA-tagged β-Arrestin-2, along with untagged modulating protein RAMP1. Activation of CALCRL stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR120S (Short Isoform) CHO-K1 β-Arrestin GPCR Assay, 2-plate Kit. (93-0924E2CP0M)
The PathHunter GPR120S eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. free fatty acids) based activation of GPR120S GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR120S and EA-tagged β-Arrestin-2. Activation of GPR120S stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Chymotrypsin Assay Kit (Fluorometric)
Chymotrypsin Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter IL17RA/IL17RC Dimerization Bioassay Kit, 10pl. (93-0999Y3-00054)
The PathHunter IL17RA/IL17RC Dimerization Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. IL-17) induced dimerization of IL17RA/IL17RC receptors. This product uses EFC technology. The cell line included overexpresses IL17RC target protein fused to PK alongside IL17RA target protein fused to EA. Upon ligand-induced dimerization of IL17RA/IL17RC, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.